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Rat liver microsomal glucose 6-phosphatase catalyses phosphoryl transfer between D-glucose 6-[(R)-16O,17O,18O]phosphate and D-glucose with retention of configuration at the phosphorus atom. Since individual phosphoryl-transfer steps appear in general to occur with inversion of configuration, this observation is most simply interpreted in terms of a double-displacement mechanism with a phosphoryl-enzyme intermediate. Such an intermediate has been proposed previously from kinetic and 32P-labelling experiments.

Original publication

DOI

10.1042/bj2010665

Type

Journal article

Journal

The Biochemical journal

Publication Date

03/1982

Volume

201

Pages

665 - 668

Keywords

Microsomes, Liver, Animals, Rats, Oxygen Isotopes, Glucose-6-Phosphatase, Glucose, Glucosephosphates, Glucose-6-Phosphate, Magnetic Resonance Spectroscopy, Molecular Conformation, Phosphorylation