Optogenetic silencing strategies differ in their effects on inhibitory synaptic transmission.

Raimondo JV., Kay L., Ellender TJ., Akerman CJ.

Optogenetic silencing using light-driven ion fluxes permits rapid and effective inhibition of neural activity. Using rodent hippocampal neurons, we found that silencing activity with a chloride pump can increase the probability of synaptically evoked spiking after photoactivation; this did not occur with a proton pump. This effect can be accounted for by changes to the GABA(A) receptor reversal potential and demonstrates an important difference between silencing strategies.

DOI

10.1038/nn.3143

Type

Journal article

Journal

Nat Neurosci

Publication Date

24/06/2012

Volume

15

Pages

1102 - 1104

Keywords

Animals, Hippocampus, Ion Pumps, Neural Inhibition, Neurons, Proton Pumps, Rats, Receptors, GABA-A, Synaptic Transmission

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