Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

© 2015 Elsevier Inc.Endolysosomes are emerging as key players that generate as well as respond to intracellular Ca2+ signals. The role of Ca2+ in modulating acidic organelle function has long been recognized, but it is now emerging that acidic organelles also act as intracellular Ca2+ stores; they actively sequester Ca2+ in their lumina and release it to the cytosol upon activation of endolysosomal Ca2+ channels. This local Ca2+ signal is crucial for endolysosomal function and/or global Ca2+ signaling. Importantly, defects in endolysosomal Ca2+ are associated with disease. This chapter discusses several complimentary approaches to monitor endolysosomal Ca2+, with particular emphasis on the inherent pitfalls that can plague the unwary.

Original publication

DOI

10.1016/bs.mcb.2014.10.031

Type

Journal article

Journal

Methods in Cell Biology

Publication Date

01/01/2015

Volume

126

Pages

159 - 195