Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

The relative structural conservation of "internal glycans" in the cell walls of pathogens suggests that they might as target epitopes less prone to variation and hence with greater potential universality as vaccine targets. Examples of such glycans include the inner core sugars of lipopolysaccharides in Gram-negative bacteria. However, due to the buried nature of such internal epitopes, this approach has been rarely adopted. Here we briefly review and compare strategic approaches and outline practical methods associated with evaluating one synergistic strategy that combines (i) blocking of the display of "external glycans" with (ii) vaccination targeted at "internal glycans."

Original publication




Journal article


Methods Enzymol

Publication Date





335 - 357


Antibacterial, Core LPS, Glycoconjugate, Sugar transporter, Vaccine, Cell Wall, Epitopes, Glycoconjugates, Gram-Negative Bacteria, Humans, Lipopolysaccharides, Polysaccharides, Vaccines