Competition-based, quantitative chemical proteomics in breast cancer cells identifies new target profiles for sulforaphane.
Clulow JA., Storck EM., Lanyon-Hogg T., Kalesh KA., Jones LH., Tate EW.
Sulforaphane is a small molecule isothiocyanate which exhibits anticancer potential, yet its biological targets remain poorly understood. Here we employ a competition-based chemical proteomics strategy to profile sulforaphane's targets and identify over 500 targets along with their relative affinities. These targets provide a new set of mediators for sulforaphane's bioactivity, and aid understanding of its complex mode of action.