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To understand how the brain functions we need to understand the properties of its constituent cells. Whole-cell patch-clamp recordings of neurons have enabled studies of their intrinsic electrical properties as well as their synaptic connectivity within neural circuits. Recent technological advances have now made it possible to combine this with a sampling of their transcriptional profile. Here we provide a detailed description how to combine whole-cell patch-clamp recordings of neurons in brain slices followed by extraction of their cytoplasm suitable for single-cell RNA sequencing and analysis.

Original publication

DOI

10.1007/978-1-0716-0818-0_9

Type

Journal article

Journal

Methods in molecular biology (Clifton, N.J.)

Publication Date

01/2021

Volume

2188

Pages

179 - 189

Addresses

Department of Pharmacology, University of Oxford, Oxford, UK.