Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Aim: To perform cross-sectional and longitudinal miRNA profiling in plasma from Duchenne muscular dystrophy (DMD) subjects and find non-invasive biomarkers in DMD. Subjects/materials & methods: Plasma was collected from 14 age and sex matched controls and 46 DMD subjects. Free-circulating and extracellular vesicle (EV)-derived miRNA expression was measured by RT-qPCR. Results: Free-circulating and EVs derived miR-29c-3p and miR-133a-3p are dysregulated in DMD subjects. Free-circulating and EV-derived miR-29c-3p are reduced in DMD subjects undergoing daily corticosteroid treatment. Free-circulating miR-1-3p and miR-122-5p are longitudinally upregulated in ambulant DMD subjects. Conclusion: We detected novel free-circulating and EV-derived dysregulated miRNAs in plasma from DMD subjects and characterized the longitudinal profile of free-circulating miRNA on plasma from DMD subjects.

Original publication

DOI

10.2217/epi-2020-0052

Type

Journal article

Journal

Epigenomics

Publication Date

11/2020

Volume

12

Pages

1899 - 1915

Keywords

Duchenne muscular dystrophy, biomarkers, corticosteroids, extracellular vesicles, microRNAs, plasma, Adrenal Cortex Hormones, Biomarkers, Circulating MicroRNA, Extracellular Vesicles, Gene Expression Regulation, Humans, Liquid Biopsy, Longitudinal Studies, MicroRNAs, Muscular Dystrophy, Duchenne