A simple method for the configurational analysis of a deoxynucleoside 5′-[16O,18O,S]phosphorothioate
Cummins JH., Potter BVL.
Methylation of 2′-deoxyadenosine 5′-phosphorothioate gives two diastereoisomeric triesters whose configurations can be assigned by 31P n.m.r. spectroscopy by methylation of an asymmetric mixture of the diastereoisomers of 2′-deoxyadenosine 5′-O- methylphosphorothioate after configurational assignment; this can be used as a simple method for stereochemical analysis of 2′-deoxyadenosine 5′-[16O,18O]phosphorothioate.