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The enzymatic transfer of activated mannose yields mannosides in glycoconjugates and oligo- and polysaccharides. Yet, despite its biological necessity, the mechanism by which glycosyltransferases recognize mannose and catalyze its transfer to acceptor molecules is poorly understood. Here, we report broad high-throughput screening and kinetic analyses of both natural and synthetic substrates of Rhodothermus marinus mannosylglycerate synthase (MGS), which catalyzes the formation of the stress protectant 2-O-alpha-D-mannosyl glycerate. The sequence of MGS indicates that it is at the cusp of inverting and retaining transferases. The structures of apo MGS and complexes with donor and acceptor molecules, including GDP-mannose, combined with mutagenesis of the binding and catalytic sites, unveil the mannosyl transfer center. Nucleotide specificity is as important in GDP-D-mannose recognition as the nature of the donor sugar.

Original publication

DOI

10.1038/nsmb950

Type

Journal article

Journal

Nat Struct Mol Biol

Publication Date

07/2005

Volume

12

Pages

608 - 614

Keywords

Crystallography, Glycolipids, Guanosine Diphosphate Mannose, Kinetics, Mannosyltransferases, Mass Spectrometry, Models, Molecular, Mutagenesis, Site-Directed, Oligonucleotide Array Sequence Analysis, Protein Binding, Protein Conformation, Rhodothermus