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We describe microarraying of carbohydrates for protein screening using either disulfide bridge or Schiff base imine immobilization chemistries on plasmachemical deposited functional nanolayers. The commonly observed issue of nonspecific background binding of proteins is overcome by spotting carbohydrates through a protein-resistant overlayer yielding spatially localized interaction with a reactive functional underlayer.

Original publication

DOI

10.1021/ja901294r

Type

Journal article

Journal

J Am Chem Soc

Publication Date

10/06/2009

Volume

131

Pages

7755 - 7761

Keywords

Aniline Compounds, Carbohydrates, Concanavalin A, Disulfides, Fluorescent Dyes, Glycols, Maltose, Microarray Analysis, Microscopy, Fluorescence, Nanotechnology, Peanut Agglutinin, Protein Binding, Spectroscopy, Fourier Transform Infrared, Sulfhydryl Compounds, Sulfonic Acids, Vinyl Compounds