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Transcriptional regulation can be established by various post-translational modifications (PTMs) on histone proteins in the nucleosome and by nucleobase modifications on chromosomal DNA. Functional consequences of histone O-GlcNAcylation (O-GlcNAc=O-linked β-N-acetylglucosamine) are largely unexplored. Herein, we generate homogeneously GlcNAcylated histones and nucleosomes by chemical post-translational modification. Mass-spectrometry-based quantitative interaction proteomics reveals a direct interaction between GlcNAcylated nucleosomes and the "facilitates chromatin transcription" (FACT) complex. Preferential binding of FACT to GlcNAcylated nucleosomes may point towards O-GlcNAcylation as one of the triggers for FACT-driven transcriptional control.

Original publication

DOI

10.1002/anie.201603106

Type

Journal article

Journal

Angew Chem Int Ed Engl

Publication Date

25/07/2016

Volume

55

Pages

8918 - 8922

Keywords

GlcNAcylation, epigenetics, nucleosomes, protein modifications, synthetic biology, Acetylglucosamine, Chromatin, Glycosylation, Models, Molecular, Nucleosomes, Protein Processing, Post-Translational