Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

D-[35S(U)]myo-inositol 1,4,5-trisphosphorothioate [( 35S]InsPS3), a synthetic, metabolically stable analogue of inositol 1,4,5-trisphosphate (InsP3), binds with high affinity (Kd 58.6 +/- 9.1 nM) to rat cerebellar membranes revealing a high density of specific binding sites (Bmax 21.5 +/- 2.1 pmol/mg of protein). Comparison with [3H]InsP3 binding reveals a higher density of sites labelled by [35S]InsPS3 and complex competition curves for displacement of specific [35S]InsPS3 by InsP3. The results suggest that [35S]InsPS3 labels two sites in rat cerebellar membranes with equal affinity: the InsP3 receptor and a site that displays low affinity for InsP3.

Type

Journal article

Journal

FEBS Lett

Publication Date

09/04/1991

Volume

281

Pages

101 - 104

Keywords

Animals, Binding, Competitive, Calcium Channels, Cell Membrane, Cerebellum, Inositol 1,4,5-Trisphosphate, Inositol 1,4,5-Trisphosphate Receptors, Inositol Phosphates, Kinetics, Ligands, Organothiophosphorus Compounds, Rats, Receptors, Cell Surface, Receptors, Cytoplasmic and Nuclear, Sulfur Radioisotopes