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The imino sugar N-butyldeoxynojirimycin (NBDNJ) is a potent inhibitor of the oligosaccharide-trimming enzyme alpha-glucosidase I. Hepatitis B virus (HBV) contains three surface proteins (HBs proteins) of different sizes that are singly or doubly N-glycosylated and are essential for the formation of infectious virus. Therefore, the replication and secretion of HBV in the human hepatoma cell line HepG2 were studied in the presence of NBDNJ. In the stably HBV-transfected HepG 2.2.15 cells and in HBV-infected HepG2 cells, NBDNJ suppressed secretion of HBV particles and caused intracellular retention of HBV DNA. The secretion of subviral particles was less affected. These data suggest that inhibitors of oligosaccharide trimming may be useful for antiviral therapy of hepatitis B and for the study of the intracellular transport of the viral glycoproteins.

Original publication

DOI

10.1073/pnas.91.6.2235

Type

Journal article

Journal

Proc Natl Acad Sci U S A

Publication Date

15/03/1994

Volume

91

Pages

2235 - 2239

Keywords

1-Deoxynojirimycin, Animals, Antiviral Agents, Blotting, Western, Carcinoma, Hepatocellular, Culture Media, DNA, Viral, Hepatitis B Surface Antigens, Hepatitis B virus, Humans, Rabbits, Transfection, Tumor Cells, Cultured, Virion, Virus Replication, Virus Shedding