BSc (Hons), PhD
My research focuses on how cells communicate with one another and the underlying molecular signalling pathways. In particular, how the levels of the intracellular messenger, Ca2+, are controlled by extracellular stimuli in both time and space.
Ca2+ signalling is implicated in myriad cellular events, from fast processes such as neurotransmission to slow processes such as gene expression. When Ca2+ signalling goes wrong, disease ensues. Moreover, Ca2+ signalling pathways can be subverted by infectious agents to their own ends. Therefore, drugs that affect Ca2+ signals may therefore correct diseases or reduce infection.
My current work examines the regulation of Ca2+ release from intracellular Ca2+-storing organelles – mainly endo-lysosomes and the endoplasmic reticulum (ER) – via the second messengers NAADP and IP3. I focus on the bidirectional cross-talk between these two organelles using a combination of molecular biology and single-cell fluorescence microscopy.
Lysosomal agents inhibit store-operated Ca2+ entry.
Morgan AJ. and Galione A., (2020), J Cell Sci
Does lysosomal rupture evoke Ca2+ release? A question of pores and stores.
Morgan AJ. et al, (2019), Cell Calcium, 86
Ca2+ dialogue between acidic vesicles and ER.
Morgan AJ., (2016), Biochem Soc Trans, 44, 546 - 553
Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) and Endolysosomal Two-pore Channels Modulate Membrane Excitability and Stimulus-Secretion Coupling in Mouse Pancreatic β Cells.
Arredouani A. et al, (2015), J Biol Chem, 290, 21376 - 21392
Expression of Ca²⁺-permeable two-pore channels rescues NAADP signalling in TPC-deficient cells.
Ruas M. et al, (2015), EMBO J, 34, 1743 - 1758