First synthetic analogues of diphosphoinositol polyphosphates: interaction with PP-InsP5 kinase.
Riley AM., Wang H., Weaver JD., Shears SB., Potter BVL.
We synthesised analogues of diphosphoinositol polyphosphates (PP-InsPs) in which the diphosphate is replaced by an α-phosphonoacetic acid (PA) ester. Structural analysis revealed that 5-PA-InsP(5) mimics 5-PP-InsP(5) binding to the kinase domain of PPIP5K2; both molecules were phosphorylated by the enzyme. PA-InsPs are promising candidates for further studies into the biology of PP-InsPs.