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Methods that site-selectively attach multivalent carbohydrate moieties to proteins can be used to generate homogeneous glycodendriproteins as synthetic functional mimics of glycoproteins. Here, we study aspects of the scope and limitations of some common bioconjugation techniques that can give access to well-defined glycodendriproteins. A diverse reactive platform was designed via use of thiol-Michael-type additions, thiol-ene reactions, and Cu(I)-mediated azide-alkyne cycloadditions from recombinant proteins containing the non-canonical amino acids dehydroalanine, homoallylglycine, homopropargylglycine, and azidohomoalanine.

Original publication

DOI

10.1002/cbic.202200020

Type

Journal article

Journal

Chembiochem

Publication Date

24/03/2022

Keywords

Bioconjugations, Bioorthogonal reactions, Dendrimers, Glycoproteins, Protein modifications