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The strategy of combined site directed mutagenesis and chemical modification with polar prosthetic groups was used to broaden substrate specificity of proteases resulting in the first successful formation of glycopeptides through the use of glucoamino acid acyl donors in yields of up to 90%.

Original publication

DOI

10.1039/b412030b

Type

Journal article

Journal

Chem Commun (Camb)

Publication Date

14/01/2005

Pages

168 - 170

Keywords

Glycolipids, Glycopeptides, Molecular Structure, Mutagenesis, Site-Directed, Peptide Hydrolases, Substrate Specificity