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Extracellular dopamine (DA) and its metabolites 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in rat nucleus accumbens were determined before and shortly following death using microdialysis. A maximal 400-fold increase in the output of DA was observed within the first 5 min of death. DA output remained elevated over the following hour at a level of approximately 70-fold above pre-death values. In contrast to that of DA, DOPAC and HVA output gradually declined. Before death the extracellular DOPAC/DA ratio was about 250; after death this ratio dropped to 0.44 at 5 min. These observations may have important implications for experiments measuring the output of (endogenous) DA and its metabolites from brain tissue in vitro: autoregulation of, e.g., transmitter release and synthesis in vitro may be seriously disrupted by the observed depletion of transmitter storage granules.

Type

Journal article

Journal

J Neurochem

Publication Date

09/1988

Volume

51

Pages

746 - 749

Keywords

3,4-Dihydroxyphenylacetic Acid, Animals, Brain, Chromatography, High Pressure Liquid, Dialysis, Dopamine, Electrochemistry, Extracellular Space, Homovanillic Acid, Male, Osmolar Concentration, Postmortem Changes, Rats, Rats, Inbred Strains, Time Factors