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A simple, inexpensive device has been designed to prepare slices or prisms of brain tumor for metabolic and release experiments by means of a hand-operated method. It is particularly suitable for very rapid slicing of small, irregular pieces of brain tissue dissected from animals or specimens available at biopsy. This simple apparatus was produced as an alternative to the more widely used motor-driven tissue choppers. A comparison of the morphological and metabolic integrity of tissue produced by the new slicer and a conventional tissue chopper is presented. The hand tissue slicer has been used to measure the release of monoamine neurotransmitters and specific neuropeptides in vitro.

Type

Journal article

Journal

J Neurosci Methods

Publication Date

02/1983

Volume

7

Pages

107 - 115

Keywords

Animals, Brain, Corpus Striatum, Culture Techniques, Dopamine, Histological Techniques, Neurophysiology, Neurotransmitter Agents, Norepinephrine, Nucleus Accumbens, Oxygen Consumption, Rats, Septum Pellucidum, Serotonin, Thyrotropin-Releasing Hormone