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To study membrane fusion mediated by synaptic proteins, proteoliposomes have been widely used for in vitro ensemble measurements with limited insights into the fusion mechanism. Single-particle techniques have proven to be powerful in overcoming the limitations of traditional ensemble methods. Here, we summarize current single-particle methods in biophysical and biochemical studies of fusion mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and other synaptic proteins, together with their advantages and limitations.

Original publication

DOI

10.1016/j.plipres.2019.01.001

Type

Journal article

Journal

Prog Lipid Res

Publication Date

01/2019

Volume

73

Pages

92 - 100

Keywords

Fluorescence Resonance Energy Transfer, Membrane Fusion, Membrane Lipids, Microscopy, Fluorescence, Proteolipids, SNARE Proteins, Single Molecule Imaging, Synaptic Transmission, Synaptic Vesicles